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The formazan product was solubilized with sodium dodecylsulfate (SDS, 10%, m v 1 in 20 mM HCl), and the absorbance was measured at 595 nm relative to the absorbance of vehicle (DMSO)-treated control cells using a multiwell scanning spectrophotometer (Victor 3 plate reader, PerkinElmer, Rodgau-Juegesheim, Germany)