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Description

After 3T3-L1 cells reached confluence (day 0), differentiation was induced in DMEM containing 10% FBS, 0.5 mM isobutyl-methylxanthine (IBMX, Sigma-Aldrich, #I7018), 1 M dexamethasone, 1 M rosiglitazone and 5 g/ml insulin for 48 h