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Note: Avoid mixing with high-concentration Vitamin C (L-ascorbic acid) or strong acids in the same step, as they may destabilize the copper ions

Looking at the reactions that use vitamin B 12 (Figure 1) , 3 an elevated methylmalonic acid level is clearly more specific for vitamin B 12 deficiency than an elevated homocysteine level

(PubMed) Kwok T, Wu Y, Lee J, et al

That said, its worth noting that the evidence supporting these uses is still limited, and more research is needed

Subsequent work by Heffernan and coworkers (2001) confirmed the peptides effects in obese mouse models, with chronic administration associated with reduced body weight gain and decreased adiposity without concurrent changes in food intake