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MCF-10A and MCF-12A were cultured in DMEM/F12 media containing all components needed for growth media (Horse serum 5%, EGF 20 ng/mL, hydrocortisone 0.5/mL, insulin 10 g/mL, Pen/Strep 1%)

Simultaneously, tumor cells undergo lipid metabolism reprogramming, producing a large amount of lipid peroxides, ultimately leading to tumor-specific ferroptosis (266)

Visca A, Bishop CT et al

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Dmitrieva et al., 2020), which is likely reflected in strong differences in 1 O 2 -responsive gene expression between the 15 and 60 min duration of the HL treatments (Figure S2A)