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In the chronic toxicity experiment, L-NAME caused severe tissue damage (myocardial and hepatic cell necrosis, necrosis of the lymphoid cells in the spleen) while L-arginine provoked predominantly congestion, edema and hemorrhages in all organs

Some evidence also exists showing that using aldosterone antagonists such as spironolactone may be beneficial, as high levels of circulating aldosterone have been associated with reduced survival time and aldosterone antagonists reduce proteinuria in man and in a small study in cats (Kai et al, 2022)

For expression in yeast, these sequences were then transferred to pYES-DEST52 vector (Invitrogen, USA) through Gateway cloning technology using LR clonase-II enzyme following the manufacturers instructions

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PMID 34706925