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PCR duplicates were removed using Picard module from GATK (v4.1.2.0) 105 , and uniquely mapped high-quality reads were collected using SAMtools (v1.11) 112 with the options (view -f 2 -q 30)

Enzymatic degradation: Microbial contamination of reconstituted solutions can introduce proteolytic enzymes that cleave peptide bonds

David|Hauser, Robert A.|Sheean, Geoffrey L

aureus and Streptococcus danieliae , which are abundant in the inflammatory skin mouse model, exacerbated skin inflammation associated with imiquimod-induced psoriasis-like dermatitis with elevated levels of TNF-, IL-17A, IL-17F and IL-22, also suggesting a potential role of gut dysbiosis in the development of psoriasis (Okada et al., 2020)