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Based on preclinical (animal and cellular) data only, inhibition of NNMT by 5-Amino-1MQ is theorised to: Raise intracellular NAD+ levels Activate SIRT1 and other sirtuins (proteins linked to metabolic regulation) Reduce fat cell (adipocyte) size and proliferation Improve insulin sensitivity These effects have been demonstrated in mouse models (Neelakantan et al., 2019), but robust human clinical trial data are currently absent

To separate parents from offspring, adult animals were picked onto freshly prepared assay plates daily until no further progeny were observed

The clearest use case is a patient on an active weight loss protocol who is struggling to maintain or build lean muscle mass

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