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Castelli, M., Friedman, K., Sherry, J., Brazzillo, K., Genoble, L., Bhargava, P

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The efficiency of transfection was estimated by GFP-dependent fluorescence and by real-time RT-PCR for total IGF-1 mRNA levels at 24 hours after transfection, as previously described 7

Similar manipulation of sensory inputs in the visual cortex, synaptic input deprivation by permanent lesion of the retina, induces the disappearance of a fraction of GABAergic boutons in few hours (Keck et al., 2011), suggesting that the rapid loss of functional inhibitory synapses may represent a general adaptive mechanism to decreased levels of synaptic activity that is conserved in different functional areas of the cortex