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Description

BCAA oxidation enzymes are present in the nuclei of PanIN lesions and PDA patient tumors The unexpected presence of the BCAA catabolic pathway in the nuclei of murine PDA cells prompted us to investigate the localization of enzymes of this pathway in mouse genetic PDA models and human PDA samples

Some of the right lungs were harvested and stored at -80C for subsequent analysis by real time quantitative polymerase chain reaction (qPCR) and western blotting, while other right lungs were perfused with cryomatrix (Thermo, New York, NY, United States) for immunofluorescence staining