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Ethics statement The protocol of animal experiments was approved by the Ethics Committee of Zhejiang Baiyue Biotech Co., Ltd., for Experimental Animals Welfare (approval number: ZJBYLA-IACUC-20220901) and carried out under the guidelines of the National Institutes of Health on Animal Care and Use

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(2025) demonstrated that inhibiting EW/CART neurons reduces alcohol intake, further underscoring their role in alcohol consumption regulation 84 .The alcohol-induced response in EWcp was postulated to be a result of signaling via GABA-A receptors, modified by 2A/D-adrenoceptors and dopamine receptors 20 , we propose that TRPA1 may also contribute 41

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