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The gradient was started at 10% B and maintained till 1 min, then linearly increased to 90% B till 3 min, maintained at 90% B till 3.5 min, then decreased to 10% B from 3.5 to 4 min, and maintained at 10% B till 5 min

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METALLURGY C07ORGANIC CHEMISTRY C07DHETEROCYCLIC COMPOUNDS C07D215/00Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems C07D215/02Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom C07D215/04Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom with only hydrogen atoms or radicals containing only hydrogen and carbon atoms, directly attached to the ring carbon atoms C07D215/10Quaternary compounds CCHEMISTRY

If additional B12 supplementation is recommended, well provide a personalised plan for you