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Biochem J 1997

The third group was incubated with N -Ethylmaleimide (NEM, a sulfite scavenger, 1 mM) for 30 min, then N -acetyl-L-cysteine (NAC, 1 mM) for 30 min to supply the endogenous generation of GSH, and DSNBD-GSH (10 M) for 30 min and imaged

Mentioned reactive aldehydes, as well as isoprostanes, particularly F2-isoprostanes, are bioactive compounds formed via nonenzymatic peroxidation of arachidonic acid (AA), serving as reliable biomarkers for assessing oxidative stress status in various clinical conditions 89

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doi: 10.1038/s41591-018-0207-3 117 VinciMBurfordAMolinariVKesslerKPopovSClarkeMet al