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Step 2: Preparation for the Procedure Once cleared for treatment, preparation for the injection involves: Hydration: Patients are often advised to hydrate well before the procedure to assist with detoxification

Acknowledgements We thank the following colleagues at the University of Sheffield for their assistance: Chris Hill for electron microscopy (carried out in the Faculty of Science Electron Microscopy Facility at the University of Sheffield), Heather Walker for the Mass Spectrometry (carried out in the biOMICS Faculty of Science Biological Mass Spectrometry Facility) and Robert Hanson for IR spectrometry

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Am J Physiol Endocrinol Metab (2020) 318:E7429

Researchers can study highly targeted interventions with minimal confounding variables