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doi:10.1016/j.freeradbiomed.2007.01.006 Bouderba S, Sanz MN, Sanchez-Martin C, El-Mir MY, Villanueva GR, Detaille D, Koceir EA (2012) Hepatic mitochondrial alterations and increased oxidative stress in nutritional diabetes-prone Psammomys obesus model

doi:10.1097/AUD.0000000000001222

His-tagged proteins were purified using Ni-NTA Agarose (Cube Biotech) and dialysed in phosphate-buffered saline (PBS) (10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , 137 mM NaCl, 2.7 mM KCl, pH 8.5), apart from SemDAPH for which a pH of 6.0 was used