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A reason for this behaviour could be that H 2 O 2 does not oxidize GSH directly, but rather through the action of glutathione peroxidase 26

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Moreover, the characteristic peak of carbonyl of Lipoid S100 that appeared in its original spectrum and in the physical mixture was shifted from 1734 cm 1 to 1636 cm 1 in BER chitosomes spectrum and this peak shift was accompanied with reduced peak intensity