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The resulting samples were centrifuged (1,600 g at 4 C) and then washed three times with PBS buffer containing 0.05% Tween 20

Leave the patches on for 3035 minutes, or until the microneedles have fully dissolved

For the animal virulence testing, data were analyzed using the non-parametric MannWhitney U test, which is more appropriate for small sample sizes and does not assume normal distribution of the data