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Transfected HeLa cells were starved for 1 h in MEM supplemented with 10 mM HEPES, pH 7.4, and subsequently radiolabeled for 3 h with 70 Ci/ml of [9,10- 3 H]palmitate (American Radiolabeled Chemicals, Inc., ART-0129-25)

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Immunity 11 , 317327 (1999)

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In this scenario, molecular histidine instead of histidine residue may introduce additional Cu 2+ /carboxyl and Cu 2+ /amino coordination, which is considered as an alternative in catalytic site optimization 33,34,35

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