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Additionally, we discovered that a GSH threshold of 10.2 nmol/mg effectively differentiates resistant cells from sensitive cells, with an AUC of 0.913, providing a robust distinction between chemotherapy-resistant and sensitive patients

[DOI] [PMC free article] [PubMed] [Google Scholar] 30.Shang Y., Li X., Liu W., Shi X., Yuan S., Huo R., Fang G., Han X., Zhang J., Wang K., et al

The PVDF membrane was then subjected to blocking with 5% milk and subsequently incubated with the designated primary antibodies overnight at a temperature of 4 C: -actin (Proteintech, 66009-1-lg) (1:50,000), SIRT1 (Abcam, ab189494) (1:1000), NF-B p65(CST, 8242) (1:10,000), NF-B p65 (acetyl Lys310) (Abcam, ab19870) (1:10,000), Type II Collagen Alpha 1 Chain (COL2A1) (Proteintech, 28459-1-AP) (1:1000), Ferritin heavy chain 1 (FTH1) (Abcam, ab183781) (1:1000), Glutathione peroxidase 4 (GPX4) (Abcam, ab125066) (1:1000), acyl-CoA synthetase long-chain family member 4 (ACSL4) (Proteintech, 22401-1-AP) (1:5000), P300 (Santa Cruz, sc-48343)(1:1000)

Sanchez-Martin, who co-reviewed with Victoria Cano-Corts, Tong Zhang, and the other anonymous reviewer(s) for their contribution to the peer review of this work

After 10 consecutive days of intraperitoneal injection, the mice in each group were weighed and then euthanized with an overdose of 3% pentobarbital sodium (180 mg/kg)