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Cells were lysed by sonication in buffer A [50 mM Tris-HCl (pH 7.5), 300 mM NaCl, 10% (v/v) glycerol, and 2 mM 2-Mercaptoethanol] supplemented with 10 mM imidazole, 0.1 mM phenylmethylsulfonyl fluoride (PMSF), and protease inhibitor cocktail (Roche cOmplete, EDTA free)

2020JH2/10300050)

Skipping doses or irregular usage reduces results

& Gulati, M

Prognostic and predictive importance of the estrogen receptor coactivator AIB1 in a randomized trial comparing adjuvant letrozole and tamoxifen therapy in postmenopausal breast cancer: the Danish cohort of BIG 198