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The LC conditions were as follows: 280 min linear gradient consisting of 432% acetonitrile in 0.1% formic acid over 260 min followed by 20 min of alternating 76% acetonitrile in 0.1% formic acid and 4% ACN in 0.1% formic acid, used to ensure all the sample had eluted from the column

Glutathione plays a pivotal role in neutralising reactive oxygen species such as free radicals and peroxides, thus protecting the cells from oxidative stress

Furthermore, by downregulating the upstream hypoxia-inducible factor-1 (HIF-1), HBO may indirectly influence the autophagic cascade, contributing to its ameliorative effects on cerebral ischemic-hypoxic injury

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