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Zebrafish Cardiac Performance Endpoint Calculation A Poincare plot was generated by plotting the time interval of each peak using the Poincare plot plug-in for OriginPro 2019 (available online: The sd1 and sd2 values generated from the Poincare plot analysis were used as the endpoint for heart rate variability

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Deficiency symptoms include anaemia, fatigue, neurological manifestations such as paraesthesia and balance problems, and glossitis, with neurological damage potentially irreversible if untreated

Injection of Lewis lung carcinoma (LLC) or B16 melanoma cells promoted tumor growth and loss of WAT and muscle (214)

Despite these promising findings, DSIP remains largely experimental, with formal clinical approval, standardized dosing, and long-term safety data still lacking