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While FFAs have acute effects on insulin secretion, there is overall less evidence that alone they impair -cell survival in acute in vitro culture settings

The expression of GPX4 was notably reduced, while the expression of ACSL4 showed a significant increase (Fig

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The cytosolic p53 inhibits the formation of autophagic vacuoles 68 by interaction with FIP200 (FAK (focal adhesion kinase) family kinase-interacting protein of 200 kDa), whereas nuclear p53 acts as a transcriptional factor of genes implied in autophagy regulation

Cells were fixed in 4% PFA and lipid droplets were stained with LipidTox (1:1000) in 1xPBS for 30 min