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This optimized preparative process achieved over a 1000-fold enrichment of the small extracellular vesicle fraction, yielding 5 mL of purified small EVs in PBS from the initial 6 L culture volume

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Enhanced tumor selectivity of 5-fluorouracil using a reactive oxygen species-activated prodrug approach

After precipitation, samples were digested overnight using LysC and trypsin (1:100 enzyme/protein ratio) at 37 C using a ThermoMixer set to 1,200 rpm