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Cells were transferred to 0.5 mL sonication tubes and lysed using a Q800R3 Sonicator (QSonica, 3 cycles at 50% amplitude, 20 seconds ON, 20 seconds OFF, at 4 C)

For an IgG Western blot to be considered officially positive under CDC criteria, the patient's blood must react to at least 5 out of 10 specific bacterial bands