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In experimental systems, the complex provides a controlled way to study copper-linked changes in collagen-associated pathways, inflammatory mediators, and oxidative stress markers

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The cell lysate was then subjected to centrifugation at 27,000 g for 40 min, followed by ultracentrifugation at 220,000 g for 1 h at 4 C to remove the cell debris and the membrane fraction from the lysis solution

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