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While controlled ROS levels are essential for normal sperm processessuch as capacitation, motility, and the fertilization mechanismexcessive ROS accumulation leads to oxidative damage, impairing sperm function and ultimately contributing to male infertility

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Sorted cells were collected in PBS with 1% BSA and RNase inhibitor, centrifuged (850 rcf, 5 min), and resuspended in cold Quenching Buffer