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To verify whether MeCbl directly engages GSDME at Cys180 in vivo, WT (GSDME WT ), C180A (GSDME C180A ) and D270A (GSDME D270A ) GSDME plasmids constructed in adenovirus vector were injected into Gsdme -/- mice via tail vein (Fig

These results, which suggest that D-Nle-Tyr-Ile-NH(CH 2 ) 5 CONH 2 is likely to be relatively hydrophobic, are in agreement with the outcome of QSAR modeling estimates generated by ADMET Predictor that calculated an octanol:water partition coefficient of 28.18 for D-Nle-Tyr-Ile-NH(CH 2 ) 5 CONH 2 (Table 6)