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Significant liver fibrosis was defined as a liver stiffness measurement 7.1 kPa

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Cryo-EM grid preparation and data acquisition Cryo-EM grids for WT and E1435Q Ycf1 were similarly prepared

Their simultaneous activation does not produce simple pathway redundancy but rather engages complementary aspects of the tissue repair response, providing the biological rationale for studying whether combination produces additive or synergistic repair outcomes exceeding either peptide alone

For a glutathionylation site to be considered for onward analysis, it was present in both biological replicates