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Concurrently, an immune response leads to the release of inflammatory mediators, resulting in an exaggerated inflammatory response that causes further damage to periodontal structures (Bitencourt et al., 2023)

Parkin is an E3 ubiquitin ligase, and its terminus contains ubiquitin-like domains as well as RING-like domains (Popov 2022)

J., Bierman, P

Briefly, the sections were permeabilized with PBS with 0.3% Triton X-100 for 30 min at room temperature, and then blocked in PBS with 0.3% Triton X-100 and 10% normal donkey serum in 0.3 M glycine for 1 h at room temperature