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Thus, the Cooling Tower Institute (CTI), the agency responsible for developing the rating system for all cooling towers, has designated that a cooling tower ton is 15,000 BTU/hr or 12,000 BTU/hr (Refrigeration Ton) + 3000 BTU/hr (Compressor Heat)

Thereafter, it should be injected once a month.

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Samples were washed in PBS and incubated with 10% donkey serum in PBS + 0.1% Triton X-100 to permeabilize the tissue and prevent non-specific antibody binding