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Dose volumes were 100 L per 20 g body weight (2) Eight weeks after chemotherapy treatment, animals were either stimulated with PMSG and sacrificed to obtain oocyte yield (see Ovarian stimulation and oocyte collection protocol below), or sacrificed without prior PMSG treatment for ovarian histology (see Follicle health and quantification protocol below), or introduced to a male of proven breeding ability for timed breeding trials (see Breeding trial protocol below)

In context to the practicality of the IU spray placement, the reader should additionally note that the physical experiments, results of which are outlined in Figure 9, were performed in a soft solid 3D-printed anatomic cast that replicated the pliability characteristics of real nasal tissues

This process may trigger the hosts innate immune system and activate the gut-joint axis, which exacerbates RA (Xu et al., 2022a)