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DC has been known to characterize node centrality in a network analysis

PubMed Kusuhara, H., Furuie, H., Inano, A., Sunagawa, A., Yamada, S., Wu, C., Fukizawa, S., Morimoto, N., Ieiri, I., Morishita, M., Sumita, K., Mayahara, H., Fujita, T., Maeda, K., and Sugiyama, Y

The PS-1_tBid_polyA plasmid was digested with NheI and BlpI to remove tBid and iRFP670 was cloned in with Gibson Assembly using the primers GibsAss_iRFP670_fwd and GibsAss_iRFP670_rev