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SLO activity was determined for each batch based on hemolytic activity, where one unit is defined as the amount of SLO required to lyse 50% of uninfected erythrocytes at 2% hematocrit in PBS for 30 min at 37 C, as determined by titration

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Conversely, G6PD inhibition lowers NADPH and NO levels, leading to EC dysfunction [39, 65, 66]

Several of these factors may also interact with each other creating a challenge when trying to determine the primary cause of sleep disturbance

ROS have a significant effect on the sperm plasma membrane and subsequent functional integrity of the sperm resulting in a loss of acrosome reaction [11], the sperm potential for fertilization [12], sperm motility and vitality [13, 14] as well as impair the sperm capacitation [15] (Figure 1)