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Description

For flow cytometry-based analysis of peptide penetration, HeLa cells were incubated with TAMRA-labeled peptides (C16, TATC16, or R11C16) at final concentrations of 10 M, 1 M, or 150 nM for 3 h at 37C and 5% CO

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Each condition included three technical replicates, and the experiment was repeated three times independently

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