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The next day, media were removed from plates, and cells were incubated with 90 L of assay buffer (1 HBSS +20 mM HEPES) + 1% Glosensor substrate for 2 h in the dark at room temperature

doi:10.3109/13697137.2014.929649

Step-by-step reconstitution process Supplies needed: 50mg GHK-Cu vial (lyophilized powder) 5ml bacteriostatic water 3ml or 5ml syringe Alcohol wipes Reconstitution steps: Clean workspace Wash hands thoroughly Wipe vials with alcohol Draw bacteriostatic water Draw 5ml into syringe Remove air bubbles Add water to GHK-Cu Insert needle through rubber stopper Slowly inject water down side of vial (not directly on powder) Aim stream at glass wall, not powder Let dissolve Remove syringe Swirl gently (don't shake) Let sit 1-3 minutes Swirl again until completely clear Inspect solution Should be clear (slightly blue tint normal) No particles or cloudiness If not clear, swirl more Label vial Write concentration (10mg/ml) Write date reconstituted Write expiration (30 days) Refrigerate immediately Store at 2-8C Protect from light Use within 30 days See our peptide storage guide , how long reconstituted peptides last in fridge , and lyophilized vs liquid peptides