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However, gaps remain in the understanding of subset functions and regulatory mechanisms: in vitro studies rely on limited cell lines (e.g., HD11 macrophages, DF-1 fibroblasts), and the lack of long-term primary immune cell culture systems hinders simulation of in vivo cellular functions in complex microenvironments

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Recent studies indicate that the gut microbiota and its metabolites are crucial for neurodevelopment, neurodegenerative diseases, and other neurological disorders (McCallum and Tropini, 2024)

For non-radioactive assays, 200 M cold ATP was used and phosphorylation was analysed by immunoblotting with phospho-specific antibodies