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Similarly, the addition of DCPIB (20 M) to isotonic PBS medium, a known and specific inhibitor of LRRC8 channels 20,21 , inhibited the production of IL-1 induced by both MSU and CPP crystals (Fig

Western blot analysis For biochemical analysis of protein activity, another half of the animals in each group were deeply anesthetized with intraperitoneal injection of ketamine (100 mg/kg) and xylazine (10 mg/kg) and sacrificed by cervical dislocation immediately

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[DOI] [PMC free article] [PubMed] [Google Scholar] 54.Hartwig A