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Importantly, TFEB has no effect on the basal transcriptional levels of downstream target genes but rather regulates their transcriptional levels in response to stimuli

K.MazucheliJ.Caparroz-AssefS

J Leukoc Biol 59(2):229240

Next, the protein was eluted using LDOX elution buffer (20 mM potassium phosphate, 200 mM NaCl, 2.5 mM d -desthiobiotin, pH 7.4) and the fractions were combined according to the recorded absorption 280 nm ( A 280nm )