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PCR products were gel-purified, then reamplified for 30 more cycles under normal conditions and gel-purified again

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Effects of Hyperoxia on Oxygen-Sensitive Transcription Factors Relevant to Aging Inhalation of 30% O 2 for 1 h followed by a return to normoxia induced a significant activation of HIF-1 (Fratantonio et al., 2021)

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Telomeres are the protective caps at the ends of chromosomes

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Timeline expectations also play into the decision

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SV calling SVs were called from WGS using MANTA and TNscope within TPO (cords-structural), with record-level and sample-level quality filters

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