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For example, the optimal combination yielded a 131-fold increase in the expression of superfolder green fluorescent protein (sfGFP), while the highest yield was achieved after single-factor optimization (TIR) of the expression of DNA glycosylase Neil3, with a threefold increase, and combinatorial optimization produced only a twofold increase [22]

The specific order of these 15 amino acids is Gly-Glu-Pro-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val

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Strianese, M., Staiano, M., Ruggiero, G., Labella, T., Pellecchia, C., DAuria, S.: Fluorescence-based biosensors