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Kuo et al., 2023

Since, in the present study, the loss of GSH due to starvation was minimal in young mice, the inability to recover from EtOH-induced GSH depletion was due to specific long-lasting effects of EtOH

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For the GSSG assay, GSH was masked by adding 20 L of 2-vinylpyridine to the neutralized supernatant, whereas 20 L of water was added for the total GSH assay