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The underlying mechanism may involve the inhibition of PKC phosphorylation in spinal dorsal horn neurons, as well as the attenuation of abnormal activation of microglia and astrocytes ( Tetramethylpyrazine (TMP, C 8 H 12 N 2 ) is the principal alkaloid isolated from the rhizome of Ligusticum chuanxiong Hort., and has been documented in the Shen Nong Ben Cao Jing for its ability to activate blood circulation and relieve pain ( 5.4 Flavonoids Pinocembrin (C 15 H 12 O 4 ) is a naturally occurring flavonoid found in plants such as Pinus cembra L., Prunus avium L., and Alpinia officinarum Hance

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