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The first sample was dissolved in methanol with 0.1 mL of 50 % of the SSA and the second sample in an ammonium buffer pH 3.5 [H 2 O/ACN (90:10) (v/v) + 1 mM NH 4 (CH 3 COO)] with CH 3 COOH

Prostate 78 , 210 (2018)

Lastly, the changes associated with cell diffusion and invasion led to the interrogation of cell stiffness where it was observed that initial GPx4 overexpression increased U251 cell stiffness from 300 to 365 Pa, however, a high level of heterogeneity prevented statistical significance ( p = 0.48, Fig

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The activity of GST and APX involve APX-GSH cycle, no significant different activity reveal that it may be not the main factor affecting GSH metabolism