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For Immunofluorescence stainings, the tissue sections were fixed with 4% paraformaldehyde (PFA) for 10 min and permeabilized using 0.1% Triton X-100 for 10 min at room temperature, followed by blocking using 3% bovine serum albumin (BSA) for 1 h at room temperature

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Such RNA interfering agents include, but are not limited to, nucleic acid molecules (including RNA molecules homologous to a target biomarker gene or fragment thereof encompassed by the present invention), short interfering RNAs (sirnas), and small molecules that interfere with or inhibit expression of a target biomarker nucleic acid by RNA interference (RNAi)

Immunotherapy is powerful, but it can be very hard on the body