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Infarct size was assessed after 30 min of ischaemia followed by 24 h of reperfusion using Evans blue staining (1%) for delineation of the ischaemic area at risk (AAR) from the non-ischaemic zone (remote area) and 1% 2,3,5-triphenyl tetrazolium chloride (TTC) staining for demarcation of the viable and non-viable myocardium within the AAR, as described 19

doi:10.1046/j.0022-202x.2001.01469.x

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Safety of intracerebroventricular copper histidine in adult rats

It has been suggested that the stronger the skin sensitizer the larger the tendency is to modify several nucleophilic sites within the same protein, as this leads to generation of a larger number of different antigens