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Unlike bulk tumor cells, CSCs exhibit stem cell-like properties, including self-renewal, differentiation plasticity, metabolic adaptability, and robust survival mechanisms under therapeutic stress

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In turn, the pool of GC is dependent on a prior reaction with glutamate cysteine ligase, requiring glutamate and CYS (Reference Peachey, Dawson and Harper51)

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The methionine synthase enzyme, which relies on B12 and Folate, is highly sensitive to oxidative stress and can become rapidly inactivated in an inflamed environment