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Moreover, apart from the unambiguous identification of the phosphopeptide sequence, the deconvoluted tandem mass spectra should contain sufficient information to localize phosphorylation sites with single amino acid resolution 1

Diabetes 50 , 18071812 (2001)

on RealSelf youll see more procedural explanations (over-resection, fat survival variance, or poor technique)

Lurz, R

Sepesi B, Corsini EM, Weissferdt A, Pataer A, Leung CH, Lin H, Le X, Mott F, Fossella F, Altan M, Tsao A, Skoulidis F, Blumenschein G, Kurie J, Zhang J, Glisson B, Lu C, Elamin Y, Antonoff MB, Hofstetter WL, Mehran RJ, Rice DC, Roth JA, Swisher SG, Vaporciyan AA, Walsh GL, Gibbons DL, Heymach JV, Cascone T