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Notably, SCN is composed of bilateral nuclei containing approximately 10,000 neurons, each of them displaying a cell-autonomous circadian oscillator

Thus, it was suggested that fluoxetine can regulate estrogen synthesis/signaling and cause dysregulation of the estrous cycle, thereby causing sexual dysfunction (Figure 3) ( Figure 3) ( 5 Effects of SSRIs on the reproductive system of aquatic organisms SSRIs are a major class of drugs of environmental concern, and fluoxetine, a psychoactive agent, is one of the best-selling prescription antidepressants in the world, which increases the likelihood of aquatic environmental contamination through municipal, industrial, and hospital discharges, potentially inducing sublethal effects in non-target species

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Therefore, affinity-purified rEE2 eluates and rMCP insoluble fractions were further subjected to size exclusion chromatography (SEC) purification: For rEE2, suitable eluates were further purified using Superdex 200 increase 10/300 or 16/600 columns (Cytiva, Little Chalfont, UK) on a GE AKTA Explorer system (GE Healthcare)

Relative expressions of target genes were analyzed using the 2Ct method with actin as the endogenous housekeeping gene