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For example, Parkin, an E3 ubiquitin ligase encoded by the PRKN gene, ubiquitinates damaged mitochondria to induce degradation and mitophagy [38], and mutations in Parkin are the most common cause of autosomal recessive early-onset PD [38]

Furthermore, TQCN activates the Nrf2 endogenous defense system, restores iron metabolism homeostasis, and reduces cellular lipid peroxidation

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The cleaved protein was loaded onto a Superdex 200 (10/300 GL) column connected to an KTA_pure device and eluted with lysis buffer