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The filtered supernatant was run over a protein A/G column (Pierce), followed by extensive washing with PBS and the antibodies were eluted with 18 mL 0.1 M glycine pH 2.5 directly captured in 2 mL neutralization buffer (1 M TRIS pH 8.7)

Captive animals were maintained under standard husbandry conditions (e.g., ad libitum food, absence of predators and competitors), which relax the constraints imposed by tradeoffs among competing functions

Growth hormone secretagogues are performance-adjacent by design

simultaneous mutagenesis of two or more contiguous amino acid positions is done using an oligonucleotide that contains contiguous N,N,G/T triplets, i.e

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